- Tissue Science and Regenerative Medicine
Session Introduction
Shaharyari Keyvan
Pasteur Institute of Iran, Iran
Title: Cloning and co-expression of Mfp3 gene from Mytilus and CsgA in Escherichia coli
Biography:
Shaharyari Keyvan is affiliated from Pasteur Institute of Iran, Iran
Abstract:
Since the identification of adhesive proteins in aquatic organisms, extensive studies on extraction and their genetic engineering for various manufacturing and medical uses have been done.
The increasing need for the rehabilitation industry for these adhesive compounds for use in surgery and restoration of tissues has caused considerable attention to be paid to studies in this field as a global platform for the use of mussels and other aquatic animals in the making of bio glue, thus the need to sea-based bioadhesives for use in the regenerative medicine has been considered.
The Mytilus galloprovincialis mussel foot protein (Mfp) includes more than eight different types of proteins that all of which include the modified DOPA amino acids after translation.
Regarding to toxicity of each of the various types of interactions with the CsgA protein, our team was able to express the Mfp3-CsgA protein in the strain of Escherichia coli called Rosetta which had a higher amount of hybrid protein expression than strains such as BL21 because of supporting disulfide bonds.
In order to optimal express, the process of this research was done with varied considering items such as temperature, type of culture medium, IPTG and shaker agitation rate.
This finding can be used for Production of bioadhesives for reconstructive medical applications including suture replacement, cardiovascular tissue repair and other clinical purposes.
Khorshid Ghazimoradi
Islamic Azad University, Iran
Title: Muscle regeneration mechanism from Pluripotent stem cells
Biography:
I am a cell biologist how work on epigenetics of stem cells and bio-photonics as my second major. I have many skills including working flow cytometry, Immune staining test and in-vivo test especially linage tracing test. I have many international publications which show me and my team quality.
Abstract:
Pluripotent stem cells are most accessible cells for regenerative medicine. This source of cells has been used in many different clinical trials for regeneration of tissues and organs from skin, kidney, eyes and many other examples. In here, we with helping of bioinformatics, investigate RNA-seq data and study the underlying mechanism of differentiation of iPScs in treatment of duchenne diseases. For this proposes the transcriptome analysis of cells after injection to duchenne muscular dystrophy patients has been determined. Furthermore, the data have been analyzed by us and its heat map1 and network2 has been established and relative mechanism has been discovered. Our analysis shows that after differentiation of iPScs several mechanisms will be activated which is consistent of biosynthesis of amino acids and protein construction. This process also activated degeneration pathway of proteins. In addition many of phagocytosis and lysis pathways have been activated. This data show not only iPScs can generate new myogenesis and myotube cells with an upregulation in protein synthesis, it also help destruction of previous structures which hinder the function of muscles.
Rodrigo Lozano
Karolinska Institute, Stockholm, Sweden
Title: Investigating tissue topographic influence on cellular phenotype with tissue engineered scaffolds
Biography:
I completed my masters research at the University of Texas at Arlington,USA, where I developed a microfluidic system to increase neuronal recordings. I completed my doctoral research at the University of Wollongong, Australia, with a thesis concerning the interaction between advanced biomaterials and novel biomedical engineering techniques, including 3D printing and electrical stimulation on neuronal, muscular and co-cultured cell types. I commenced my first postdoctoral research position at the Karolinska Institutet in Sweden. In the Katajisto Lab, I have had the opportunity to work in new areas of research such as stem cells, ageing, and with intestinal organoids. My primary project concerned the development of artificial intestinal scaffolds for the purpose of investigating the influence of intestinal tissue topology on stemness.
Abstract:
The human small intestine possesses a distinct villus-crypt topography in which differentiated cells reside in the villi whereas the proliferative cells reside within the crypts. Each crypt contains multiple stem cells that are interspersed between Paneth cells in a specific pattern along the bottom of these crypts. Paneth cells are a crucial part of the intestinal stem cell niche and, in response to multiple stimuli, they supply the stem cells with cues guiding their activity and maintenance. Both stem and Paneth cell phenotypes are related to their localization in these crypts. However, it is not fully understood why stem and Paneth cells are located at the very bottom of these crypts, and whether such localization is necessary for their maintenance and function. In pursuit of these questions, bioengineered scaffolds containing crypt-villus-like topography that replicates key features were fabricated using different biomaterials, including hydrogels. With these bioengineered scaffolds we are addressing the role of mechanical forces, and extracellular matrix constituents in maintaining crypt function and intestinal tissue renewal. Our ultimate goal is to provide insight into whether their localization is an important factor for the stem cell identity and function.
Pranab Roy
Haldia Institute of Technology, India
Title: Characterization of plant growth promoting bacteria isolated from nodule co-infecting a single leguminous plant
Biography:
Having completed his academic studies from the best institutions in India, securing 1st class in all examinations, Prof. Roy had post- doctoral research experience at Yale University School of Medicine and University of Connecticut Health Centre, USA working on advanced molecular biology. He joined as lecturer in Bose Institute where he characterized a novel repetitive DNA sequence by molecular cloning and sequencing when these techniques were just being introduced in India in mid 80’s. He joined industrial R&D to set up the genetic engineering unit at Hindustan Lever Research Centre, Mumbai. The functioning of the unit is reflected in the number of publications arising out of the work carried out there and the sister concern, Unilever Research Labs. Prof. Roy also did pioneering work with Tea Quality during his tenure at Tea Research Association, Jorhat. This led to the patent on optimization of fermentation time for Black Tea Production.During his tenure at Burdwan University since the inception of Biotechnology department, Prof. Roy was instrumental in establishing the Department
Abstract:
The green revolution ushered in a growth in the production of food crops in India. From a net importer of food grains ,India became self-sufficient increasing the total production from 50 million metric tonnes in mid-sixties to 200 million metric tonnes in mid eighties. This was possible due to higher yielding and hybrid varieties of seeds, increased input of chemical fertilizers and insecticides or pesticides.
However , the pitfalls of high usage of chemical fertilizers was a deterioration in the quality of agricultural soil. The natural microbes present in the rhizospheric soil died due to excess chemicals the depletion of oraganic carbon content. The water retention capacity was also adversely affected due to lower porosity of the soil, sometimes the soil became acidic causing lower productivity of plants.
To overcome all these problems, the use of organic manure and biofertilizers were introduced. These are beneficial to the crop plants due to the following properties:
1.Nitrogen Fixation
2. Inorganic phosphate solubilization.
3. Production of siderophore.
4.Production of ACC Deaminase.
Nitrogen fixation by symbiotic microorganisms like Rhizobium,ocuring in the root nodules of leguminous plants have been reported long back.
In our studies, aspetic root nodules of Fenugreek (Methi) were crushed to isolate a variety of nitrogen fixing microbes. Though microbiological and biochemical studies indicated these mucoid colonies all gram negative with different morphologies to the Rhizobium species, sequencing of 16s rRNA genes and molecular phylogeny showed three o f the isolates to be :
- R1 Enetrobacter cloacae (KX687556)
- R10 Pantoea dispersa (KX687557)
- R12 Enterobacter ludwigii (KX687554)
The temperature and salinity tolerance of these cultures were studied.In order to increase these properties of abiotic stress tolerance. UV Mutagenesis was tried , varying the time of exposure of the plated cultures to UV radiation, the survivors (>1% of initial population) were screened for growth at 0.5M NaCl and at 44C.
The selected mutants of R1 ,R10 and R12 were studied for the plant growth promoting activities like; IAA (growth hormone) production., Siderophore production, Phosphate solubilization .
Finally the effects of the isolated bacteria and mutants of each and a consortium of all three were pre-treated with methi seeds and germinated in pots to find out their effects on the growth of the plants. These are being analyzed after 40 days of growth in the soil.