10th International Conference on Tissue Science and Regenerative Medicine
Tokyo, Germany
Shaharyari Keyvan
Pasteur Institute of Iran, Iran
Title: Cloning and co-expression of Mfp3 gene from Mytilus and CsgA in Escherichia coli
Biography
Biography: Shaharyari Keyvan
Abstract
Since the identification of adhesive proteins in aquatic organisms, extensive studies on extraction and their genetic engineering for various manufacturing and medical uses have been done.
The increasing need for the rehabilitation industry for these adhesive compounds for use in surgery and restoration of tissues has caused considerable attention to be paid to studies in this field as a global platform for the use of mussels and other aquatic animals in the making of bio glue, thus the need to sea-based bioadhesives for use in the regenerative medicine has been considered.
The Mytilus galloprovincialis mussel foot protein (Mfp) includes more than eight different types of proteins that all of which include the modified DOPA amino acids after translation.
Regarding to toxicity of each of the various types of interactions with the CsgA protein, our team was able to express the Mfp3-CsgA protein in the strain of Escherichia coli called Rosetta which had a higher amount of hybrid protein expression than strains such as BL21 because of supporting disulfide bonds.
In order to optimal express, the process of this research was done with varied considering items such as temperature, type of culture medium, IPTG and shaker agitation rate.
This finding can be used for Production of bioadhesives for reconstructive medical applications including suture replacement, cardiovascular tissue repair and other clinical purposes.