9th International conference on Tissue Science and Regenerative Medicine November 12-13, 2018 Singapore

Biography:

Studies of oral fungal have indicated that prevalence of Candida was significantly higher in diabetics both in healthy controls and complete denture wearers compared to non -diabetics. The aims of study was to determine the prevalence oral Candidiasis among both diabetics and non–diabetics patients and evaluate the contribution of risk factors with the prevalence and distribution of Candida species with oral Candidiasis.

Abstract:

The present investigation was carried out in the department of medical microbiology and clinical samples were collected through regular visits to three main hospitals Al-Noor, Al-Amean, Al-Thawra and diagnosis was in the ALFA medical laboratory in Ibb City during period from January into November 2016 .In the current study 70 oral swabs samples were collected from surfaces of the upper of oral cavity and denture of all patients and then placed into a test tube containing 5ml subouraud's dextrose broth. The positive sample was processed for Gram's staining, Germ tube test, Chlamydospores test, Carbohydrate utilization medium examination and Chromagar test was made for differentiation between Candida a species. The results in this study showed that prevalence of Candida oral infection in Ibb City was 36(52%) positive with oral candidiasis out of 70 samples studied while, 34(48%) of patients studied were negative with oral candidiasis. On the other hand, the results also showed the distribution of oral Candida infection among diabetes and non-diabetes patients was 29(41%) and 7(11%) respectively, this results concordant with many previous studies. Candida albicans was the most prevalent types of Candida a species in this study. The results in current investigation also showed a significantly relationship between crews users, diabetes patients, age and oral candidiasis.

Biography:

Communicable Disease Control, Directorate General of Health Services, Ministry of Health and Family Welfare, Bangladesh

Abstract:

Bangladesh has reduced the death of rabies case-patients by 50% over the last few years. Clinical and epidemiological knowledge of human rabies is crucial to direct further research investigation and to implement disease control measures.

Methods: We used data from the rabies record book of the Infectious Disease Hospital (IDH), Dhaka, Bangladesh from 2006 to 2017. We reviewed the medical records of 422 patients reported from 2011 to 2015 who had a clinical diagnosis of rabies.

Results: We found a strong trend of declining human rabies cases in Bangladesh from 2006 to 2017 (p<0.001 and tau = -0.85). In Sen’s slope test we found, over the 12 year period the slope changes at the rate of 11.45 (95% CI: -14.00 to -8.00) annually (p<0.001). The declining trend of human rabies cases can be explained by the number of dogs being vaccinated against rabies during 2011 to 2017 (adjusted R2=0.55). Dogs with unknown vaccination status, comprised the majority of exposures (n=380, 90%) followed by cat (n=24, 6%), jackal (n=12, 3%) and mongoose (n=6, 1%). The majority (n=327, 78%) of the victims sought treatment from traditional healers and 12% (n=51) received post-exposure prophylaxis (PEP). There was a difference in incubation times between the sites of bites in which the patients were exposed. The shortest, longest and median reported incubation periods were 11, 1080 and 52 days respectively. Cases with a wound on the head and neck appeared to have a shorter incubation time than those with wounds at other sites. Clinical symptoms included hydrophobia (97%), acrophobia (84%), photophobia (10%), and hypersalivation (7%). The case-fatality rate was 100%.

Biography:

Research areas mainly   infectious diseases like Leprosy, and Tuberculosis I am working in this Institute since last more than 30 years I have done MSc., M.Phil & Ph.D from Agra College, throughout Ist class I am  guiding Ph.D,students Project and Training students who wish to do research work in biomedical fields.I  got  an  opportunity  to take  my  basic training by eminent electron microscopistProf. M. Nishiura, from Kyoto University, Japan for more than 2 years. I also privileged to take the training on “Cell Ultra structural   and Electron Microscopy” under Prof. B.K.Ghosh, Ruttgers University, New Jersey, U.S.A. in WHO workshop.

Abstract:

Electron microscopy used to investigate the detailed structure and configuration of the mycobacteria which may lead to an understanding the role of  infections  and in transmitting the diseases. In the natural world more than 99% of bacteria exist as biofilms and according to NIH report more then 68% of all human infections are associated with  biofilms formation. Biofilms are slimy, glue-like substance that excreted by bacteria and aggregate on living surface. Biofilms are formed to protect the bacteria from host defences, antibiotics and from harsh environmental conditions. Biofilms are found almost everywhere in nature, including soil, water pipes, and even inside the human body. Many Mycobacteria  can develop biofilm, a multicellular structure largely combining bacteria and their extracellular polymeric substances (EPS). The formation of biofilm results in an alternative existence in which microbes ensure their survival in adverse environments. Bacterial biofilms are often associated with  infections specially with medical implants such as catheters and other medical devices. Attachment of mycobacteria involved in biofilm formation in the liquid air interface is a complex process, with many variables such as pH, nutrient levels, iron, oxygen, ionic strength and temperature, affecting the outcome.

We had taken two mycobacterial species for study of Mycobacterial biofilm. The isolates were subcultured and characterized biochemically and molecularly. M.avium developed strong amount of biofilm at 30oC and 42oC  as compared to 370C. M tuberculosis (H37Rv) developed strong biofilm at 370C  and no biofilm at 300C and 420C in MB 7H9 media and Sauton’s media. The selected non tuberculosis mycobacteria  and  H37Rv developed strong biofilm in the presence of OADC enrichment in MB7H9   as well as Sauton’s medium. Isoniazid showed strong inhibited  biofilm in fast grower and sensitive isolates. However, Pyrazinamide and Isoniazid  inhibited  biofilm of M.tuberculosis (H37Rv) and in MDR isolates Ethionamide and Moxifloxacin inhibited biofilm in slow grower and fast grower Mycobacteria. However, many  mycobacterial species are known to form biofilms, little is known about either  the genetic requirements, patterns of gene expression. In micro array hybridisation we have found that six genes were  expressed in M.avium.  In M. tuberculosis MDR isolates seven genes were expressed and two genes Rv0359 and Rv3526 were homologus as earlier reported in  P. areuginosa and M. avium  which might be responsible for biofilm formation.

Biography:

Medical Biotechnology undergraduate with a grounded background in biological sciences of various sub-disciplines, rooted in a strong passion in understanding complex life processes and its utilisation for human benefit. Holds a substantial scope of scientific research experiences and is continuously building up efficiency through experience both in the field and within laboratory settings.
 

Abstract:

Background: Neuroinflammation is a primary risk factor of neurodegenerative diseases (ND), with microglia cells under pathological conditions directly contributing to neuroinflammation. Induced autophagy has been known to therapeutically reduce neuroinflammation without exacerbating the pathological condition of the disease. Existing treatments for inducing autophagy in neuronal setting are few but effective, with some noted to have reached clinical trials phase II, and much scientific support for new compounds to modulate autophagy in a neuronal setting. Hence, this study focuses on the autophagic inducing potential of orientin on lipopolysaccharides-stimulated BV2 microglial cells. Methodology: BV2 microglia cells pre-treated with orientin at maximum non-toxic dose or MNTD (15 µM) and ½ MNTD (7.5 µM), for a 3-hour period, followed by induction of neuroinflammation via 0.1 μg/mL of lipopolysaccharide (LPS) stimulation. Autophagolysosome production was qualitatively determined with Acridine Orange (AO) staining and expression of autophagy pathway proteins were analysed via Western Blot analysis. Results: The induction of intracellular autophagolysosomes, under MNTD and ½ MNTD treatment of orientin qualitatively determined by AO staining confirmed the near completion of autophagy, with particular noteworthy observation of low complete neuronal death. Western Blot results showed upregulation of autophagy proteins Beclin-1, ATG5 and LC3-II, highlighting upregulation of key autophagy pathways in autophagy vacuole formation. Conclusion: Orientin possesses significant likelihood of contributing to field of autophagy inducing therapeutic agents for targeting neuroinflammation in neurodegenerative diseases. Its autophagy inducing properties most likely stem from its ability to directly affect the mTOR signaling pathways by downregulating PI3K-I/Akt and MAPK/Erk 1/2 signaling pathway, encouraging future studies are required to refine this hypothesis.

Biography:

Having completed his academic studies from the best institutions in India, securing 1st class in all examinations, Prof. Roy had post- doctoral research experience at Yale University School of Medicine and University of Connecticut Health Centre, USA working on advanced molecular biology. He joined as lecturer in Bose Institute where he characterized a novel repetitive DNA sequence by molecular cloning and sequencing when these techniques were just being introduced in India in mid 80’s. He joined industrial R&D to set up the genetic engineering unit at Hindustan Lever Research Centre, Mumbai. The functioning of the unit is reflected in the number of publications arising out of the work carried out there and the sister concern, Unilever Research Labs. Prof. Roy also did pioneering work with Tea Quality during his tenure at Tea Research Association, Jorhat. This led to the patent on optimization of fermentation time for Black Tea Production.During his tenure at Burdwan University since the inception of Biotechnology department, Prof. Roy was instrumental in establishing the Department 

Abstract:

The green revolution ushered in a growth in the production of food crops in India. From a net importer of food grains ,India became self-sufficient increasing the total production from 50 million metric tonnes in mid-sixties to 200 million metric tonnes in mid eighties. This was possible due to higher yielding and hybrid varieties of seeds,  increased input of chemical fertilizers and insecticides or pesticides.

 However , the pitfalls of high usage of chemical fertilizers was a deterioration  in the quality of agricultural soil. The natural microbes present in the rhizospheric soil died due to excess chemicals the depletion of oraganic carbon content. The water retention capacity was also adversely affected due to lower porosity of the soil, sometimes the soil became acidic causing  lower productivity of plants.

To overcome all these problems, the use of organic manure and biofertilizers were introduced. These are beneficial to the crop plants due to the following properties:

1.Nitrogen Fixation

2. Inorganic phosphate solubilization.

3. Production of siderophore.

4.Production of ACC Deaminase.

Nitrogen fixation by symbiotic microorganisms like Rhizobium,ocuring in the root nodules of leguminous plants have been reported long back.

In our studies, aspetic root nodules of Fenugreek (Methi) were crushed to isolate a variety of nitrogen fixing microbes. Though microbiological and biochemical studies indicated these mucoid colonies all gram negative with different morphologies to the Rhizobium species, sequencing of 16s rRNA  genes and molecular phylogeny showed three o f the isolates to be :

1. R1 Enetrobacter cloacae (KX687556)
2. R10 Pantoea dispersa (KX687557)
3. R12 Enterobacter ludwigii (KX687554)

The temperature and salinity tolerance of these cultures were studied.In order to increase these properties of abiotic stress tolerance. UV Mutagenesis was tried , varying the time of exposure of the plated cultures to UV radiation, the survivors (>1% of initial population) were screened for growth at 0.5M NaCl and at 44C.

The selected mutants of R1 ,R10 and  R12 were studied for the plant growth promoting activities like; IAA (growth hormone) production., Siderophore production, Phosphate solubilization .

Finally the effects of the isolated bacteria and mutants of each and a consortium of all three were pre-treated with methi seeds and germinated in pots to find out their effects on the growth of the plants. These are being analyzed after 40 days of growth in the soil.

Biography:

Ilknur Tuncer was born in Turkey, 1980. She received two BSc degrees in chemistry and genetics from Bogazici University, Turkey, MSc degree in marine sciences from Bangor University, UK and PhD degree in marine sciences from Dokuz Eylul University, Turkey. She has several books, publications. She is an editorial member for several scientific journals. She joins conferences as committee member and speaker. Her current reseach interests are marine environment, microbiology, phylogeny, species-area relations, human health, medicine.

Abstract:

Phylogenetic diversity of isolated bacteria and biogeochemical variability of sediments in shallow and deep basins of Eastern Mediterranean Sea were studied. According to 16S rRNA gene sequence analysis, the strains isolated from the study area were belonged to the Firmicutes, Gammaproteobacteria and Actinobacteria. The present study provided highly diverse bacterial strains of fourteen different families especially with probability of representing new taxa in addition to the dominant genus Bacillus in each sediment samples. The actinomycetes were tested physiologically using commercial kits and found that they utilized proteins rather than carbohydrates. Geochemical parameters of the sediments together with enzymatic activity results of the strains underlined the nitrogen limitation in the area. The highest antibiotic resistance for the Firmicutes and Gammaproteobacteria strains was obtained from coastal sediments compared to deep basins supported by a positive correlation between sediment size and antibiotic resistance. The influence of environmental parameters was observed as the variability of bacterial community composition such that phylogenetic diversity was much higher in higher taxa for shallower 

regions, especially in North Aegean Sea as compared to deep basins of Eastern Mediterranean Sea as supported in hierarchical cluster analysis of the isolates and statistical analysis including principe component analysis

Biography:

Because multi-drug resistant Gram-positive bacteria have been isolated frequently worldwide and have difficulties in the treatment, development of new antibiotics and alternative treatment choices are required. Combination antibiotherapies have a distinct advantage over monotherapies in terms of their broad spectrum and synergistic effect. Antibiotic combinations are sometimes in an attempt to prevent or delay the in vivo emergence of drug-resistant subpopulations of pathogenic organisms. In the present study, it was aimed to investigate the in vitro activity of linezolid and dalbavancin in combination with vancomycin against clinical VRE strains.

Abstract:

A total of 30 randomly-selected clinical VRE strains were included in the study. Each strain was isolated from different patients who were admitted to the different clinics of the university’s hospital. Bacterial identifications of the strains were undertaken using conventional routine methods. The antibiotics used in the study were vancomycin, linezolid and dalbavancin. Resistance to agents tested was investigated by using microbroth dilution method as described by Clinical and Laboratory Standards Institute (CLSI). They were prepared in accordance with CLSI proposals. Cation adjusted Mueller-Hinton broth (CAMHB) was used for all experiments. Additionaly, in the tests for dalbavancin, CAMHB with 0.002% final concentration of polysorbate 80 was used. The inoculum of strain was adjusted to approximately 5x105 cfu/mL in the wells. The minimum inhibitory concentration (MIC) was defined as the lowest concentration of antibiotic giving complete inhibition of visible growth, and interpreted in accordance with the CLSI guidelines. Quality control of the test procedures was monitored via testing of the reference strains S. aureus ATCC 29213 and E. faecalis ATCC 29212.

In vitro activities of antibiotics in combination were assessed using a microbroth checkerboard. The fractional inhibitory concentration (FIC) indexes were calculated using the following formula: FICI=FICA + FICB. The FICI was interpreted as follows: synergism, FICI ≤0.5; additive / indifference, FICI ˃0.5-≤4; antagonism, FICI ˃ 4.

Biography:

TBA

Abstract:

To explore the case management and disease burden of bacterial meningitis among children, admitted to Benghazi hospital from (January 2014 - December 2015), to study the trends of bacterial aetiology amongst cases of meningitis. and to isolate, identify and determine the antibiotic susceptibility patterns of pathogens associated with bacterial meningitis Cerebrospinal fluid (CSF) samples were collected from children admitted to children hospital units aged from few days up to 13 years old, and suspected to have bacterial meningitis, Doctor will almost always do a lumbar puncture.  In this retrospective study, the data were collected from the laboratory records and statistical unit. A total of 6306 CSF children samples from clinically suspected case of meningitis were subjected to microbiologic unit. There were (400) cases clinically diagnostic as BM in children, 103 cases were found to be CSF bacterial culture positive and (77) were positive by gram stain (41%). Most of meningitis cases were found in male’s children 225 (56%) than females175 (44%). The ratio of male to female was (1.3-1). Fever was the most common presentation (90%) followed by vomiting (60%), poor oral intake (51%). The mortality rate was (7%), Coagulase negative staphylococcus was the most frequent pathogens isolated (55%)followed by E. coli (15.5%)then staph aureus (14.6), followed by streptococcus pneumonia (11.7%), Klebsiella pneumoniae (9.7%), Acinetobacter species, (5.8%), then Pseudomonas species, (4.9%), and among the seasonal effect the highest incidence in the study area were occurred on Augustus.

Biography:

Prof. Andrzej Lange, MD, FRCP graduated with a medical degree with distinction from the Medical School in WrocÅ‚aw, Poland is a professor in the Institute of Immunology and Exp Therapy of the Polish Academy of Sciences and a founder and head of the Lower Silesian Center of Cellular Transplantation in WrocÅ‚aw. His international experience started in 1973 – 1974 as a Leverhulme Fellow in the Middlesex Hospital Medical School, London. He has been a visitor and lectured in a number of European and North American scientific institutions. Known from his activity in the field of bone marrow transplantation and regenerative medicine.  

Prof. Lange was awarded with several scientific distinctions and served to a number of National and European institutions being also active in co-editing journals.  He is an author and co-author of 249 scientific papers, in peer-reviewed journals with a cumulative IF of 270 in the years 1995–2017. 

Abstract:

Biography:

I am Iranian scientist. I did my PhD. and Research work on Photochemistry or chemistry (Ag). And up to now my personal life for doing research with our co-worker on cultivation, extraction, formulation and produce natural products out of medicinal and Aromatic plants. So we designed research apparatuses and pilot of essential oil distillation and herbal extraction. Published 6 Book in Persian language and international of two chapters of bio-activity on herbal extracts or essential oil. Finish lots of thesis with different topics on Msc. and PhD. with my students. Publish many papers on this topic in national and international (ISI) journals. Know my position are as scientist in Research Institute Forests and Rangelands (RIFR) -Tehran-IRAN, Chairman of "Union Medicinal plants of Iran" (UMPI) and chair member of "Iranian medicinal plants society" (IMPS). I got three national award abut selected and important medicinal plant as natural product or instead of synthetic medicine and bio-logical uses. And so on…

Abstract:

The increasing demand in finding novel natural compounds or product in the world has diverted scientist’s attention toward of this sources. The very diverse environmental conditions and the rich variety of natural resources made it possible for the Iranians to create a great and flourishing civilization. Iran has hi ranks in medicinal and aromatic plant in natural resource and cultivated manner, traditional knowledge and producing of natural products in the world spatially in Middle East. Iran also is very famous in producing essential oil and water extraction from national plants spatially Rosa damacena, menthe spp and Thym spp. The methods of extraction in Rosa damascena cultivated in extensive zone of Iran are shown different constitution. All Samples were analyzed by GC and GC/MS.  The main constituents of oil extracted traditionally, samples were extracted by two hydro distillation method which designed by authors in Research Institute of Forests and Rangelands. Main components of essential oils were citronellol, cis-p-menth -2-en-1-ol and geraniol. These compounds and others are very popular in producing drug anti-cancer, anti-bacterial out of that and we should take care of over doses or toxicology. From my research work, going to presenting the natural constituents and traditionally patients consuming herbal applied and natural ways to controlling different Bacteria growing , as every scientist now reach to the point most of microbes and fungi or…. Become resistant to chemicals uses. So in this way we selected plants and main compounds out of them and got it best results. So it is necessary for a protected herbals and product to be provided with information on its ingredients, indications, dosage, pharmacology, contraindications and possible side effects before a rational decision on its use.

Biography:

For 25 years, he has been developing gene and cell therapy using non-human primates, immune-tolerant mice and rats to protect against the side effects of radiation. He collaborates with clinicians to develop  strategies for treatment of patients after radiotherapy overexposures. He has participated in the first establishment of proof of concept of the therapeutic efficacy of Mesenchymal stem cells (MSCs) for the treatment of hematopoietic deficit, radiodermatitis and over dosages of radiotherapy. He has contributed to the first reported correction of deficient hematopoiesis in patients (graft failure and aplastic anemia) thanks to intravenous injection of MSCs restoring the bone marrow microenvironment, mandatory to sustain hematopoiesis after total body irradiation. He is scientific investigator of Clinical phase II trial evaluating the efficacy of systemic MSC injections for the treatment of severe and chronic radiotherapy-induced abdomino-pelvic complications refractory to standard therapy (NCT02814864Hirsch Index 26.

Abstract:

The late adverse effects of pelvic radiotherapy concern 5 to 10% of them, which could be life threatening. However, a clear medical consensus concerning the clinical management of such healthy tissue sequelae does not exist. Our group has demonstrated in preclinical animal models that systemic MSC injection is a promise approach for the medical management of gastrointestinal disorder after irradiation. We have shown that MSC migrate to damaged tissues and restore gut functions after irradiation.

The clinical status of four first patients suffering from severe pelvic side effects resulting from an over-dosage was improved following MSC injection in a compassional situation. A quantity of 2x106 - 6x106 MSC /kg were infused intravenously to the patients. Pain, hemorrhage, frequency of diarrheas and fistulisation as well as the lymphocyte subsets in peripheral blood were evaluated before MSC therapy and during the follow-up. Two patients revealed a substantiated clinical response for pain and hemorrhage after MSC therapy. In one patient pain reappeared after 6 months and again substantially responded on a second MSC infusion. A beginning fistulisation process could be stopped in one patient resulting in a stable remission for more than 3 years of follow-up. The frequency of painful diarrhea diminished from an average of 6/d to 3/d after the first and 2/d after the 2nd MSC injection in one patient. In all patients, prostate cancer remained in stable complete remission. A modulation of the lymphocyte subsets towards a regulatory pattern and diminution of activated T cells accompanies the clinical response in refractory irradiation-induced colitis. No toxicity occurred.

MSC therapy was safe and effective on pain, diarrhea, haemorrhage, inflammation, fibrosis and limited fistulisation. For patients with refractory chronic inflammatory and fistulising bowel diseases, systemic MSC injections represent a safe option for salvage therapy. A clinical phase  II trial will start in 2018.

Biography:

Jaeseo Lee is a MS candidate at the Kyung Hee University, and she began researching in the Kwon lab in Jan of 2016. She has her expertise in tissue engineering and biomaterials. She is working to research drug delivery system using graphene quantum dots. She is also leading a project to develop drug delivery vehicles to treat specific breast cancer.

Abstract:

Graphene Quantum Dots (GQD) have been widely used for theragnosis for cancer disease. However, one of the current challenges is considerably difficult to release therapeutic agent to target site. In this study, we modified GQD that introduced herceptin through polyethylene glycol (PEG) and disulfide bond to induce active targeting for specific cells and control the drug release. The resulting of UV spectroscopy, Fluorescence, Fourier Transform Infrared showed that GQD-complex (GQD-comp) was conjugated successfully. The cell viability was presented that GQD-comp had a substantial effect on HER2-overexpressing breast cancer cells. In endosomal condition of low pH and high glutathione, doxorubicin was rapidly released from Doxorubicin loaded GQD-comp. Besides, this optical imaging by confocal laser scanning microscopy indicated to certainly confirm intracellular trafficking. In conclusion, GQD-comp had potential theragnostic agents to HER2-positive breast cancer cells. 

Biography:

Donghyun Lee is a Ph.D. candidate at the Kyung Hee University, and he began researching in the Kwon lab in May of 2013. He has his expertise in tissue engineering and biomaterials. He is working to research biological properties of gelatin hydrogel and gold nanoparticle with the goal of designing injectable material systems for bone regeneration. He is also leading a project to develop biomaterial to treat alveolar bone defects. He has published 4 scientific research papers as a first author and 18 papers as a co-author.

Abstract:

Poly(lactide-co-glycolide) (PLGA) is a biocompatible and biodegradable polymer that has been widely used in devices for tissue engineering and drug delivery applications. Gold nanoparticles (GNPs) have also been used as biomaterials and have been found to have a positive effect on bone formation. In this study, we synthesized thiol end-capped PLGA (PLGA-SH) and used it for binding GNPs. This PLGA was processed into a sheet form via electrospinning. GNPs with an approximate size of 30 nm were attached onto the PLGA-SH sheet surfaces (PLGA-GNPs). This membrane was characterized by thermogravimetric analysis, ultraviolet/visible spectrophotometry, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, and confocal laser scanning microscopy. Characterization results show that the GNPs are well attached on the PLGA-SH sheet and it is possible to control the GNPs load. Additionally, in-vitro results showed that PLGA-GNPs have good biocompatibility. They were also found to enhance osteogenic differentiation of human adipose derived stem cells. From these results, we have determined that the PLGA-GNP fibers can be useful as materials for bone regeneration and can also potentially serve as drug carriers.

Biography:

TBA

Abstract:

Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies worldwide and one of the main causes of cancer-related mortality globally. Its incidence is increasing at alarming rates. The presence of cirrhosis is the major risk factor and this is largely due to chronic HCV and HBV infection. Serum alpha-fetoprotein (AFP) has insufficient sensitivity and specificity for detection of hepatocellular carcinoma (HCC). Estimation of serum level of certain up regulated proteins as glypican-3 (GPC-3) in HCC could be a  promising biomarker for screening and early HCC detection. Recently glypican-3 (GPC-3) was suggested as a novel  biomarkers for the detection HCC.

Biography:

TBA

Abstract:

The purpose of study is to explore the efficacy and safety of platelet rich plasma (PRP) in the non-operative management of shoulder tendinopathy amongst individuals with spinal cord injury.

In this case in road traffic accidents that cause spinal injuries to the central nervous system, which increased morbidity and motility. The complications in patients were developed, resulting to a challenging problem for medicine. Platelet is naturally involved in haemostasis and tissue healing processes due to their content in growth factor and other bioactive molecules. Basic science and preclinical evidence supports the use of platelet derived growth factors as well as of PRP for enhancing reparatory processes in spinal cord injury.

In recent advancement in the field of regenerative medicine it was thought that this would grant a new approach to this problem, as it had proven beneficial in the repair of peripheral nerve injuries and their regeneration. This study was done to evaluate the effect of Platelet Rich Plasma (PRP) and its effect on the spinal cord post trauma. Subsequently, it was seen that PRP proved beneficial with marked positive effects in both muscle tone and muscle control and marked clinical improvement, although it can still be said that further research must be done in this field.

Clinical results about the use of PRP for bone, tendon, cartilage or spinal cord healing are encouraging and continue to accumulate in the recent years. Proteomic profiling and biomarker based PRP characterization have the potential of advancing the field of PRP application. High quality studies are awaited in order to enable clear cut therapeutic indications.