Bipasha Bose
Yenepoya University, India
Title: CD34+/CD45- stem cells from diverse lineages and their applicability for cell therapy
Biography
Biography: Bipasha Bose
Abstract
From the perspective of cell therapy and understanding the basic biology of adult stem cells residing in tissues of the different germline origin, we have isolated and characterized CD34+/CD45- stem cells from various organs, other than bone marrow. These studies were aimed at establishing a proof of concept for the translational applicability of such cells in preclinical animal model system. Till now, in clinical scenario, bone marrow has been the exclusive source for CD34+/CD45- for all the therapeutic applications. The advantage of using other cell types for obtaining CD34+/CD45- is avoiding the invasive bone marrow collection procedure. The presence of CD34+/CD45- cells in liver (endoderm), skin (ectoderm) and muscle(mesoderm) were established using immunofluorescence staining. The CD34+/CD45- were isolated from liver, muscle and skin using FACS sorting. Also, CD34+/CD45- cells from muscle had proven to ameliorate muscular dystrophy in mouse model of muscular dystrophy.