Stepanova O V works in the field of the Regenerative Medicine. She studies the resident stem/progenitor cardiac and neural cells. She demonstrated that the heart of the patients with heart failure due to dilated cardiomyopathy contains the progenitor resident cardiomyocytes and dedifferentiated cardiomyocytes. These cardiac cells possibly can proliferate and differentiate to mature cardiomyocytes and recover heart function and structure after injury. She found that myosin activating protein kinases may contribute in myofibril formation during the cardiomyocyte differentiation. Now she is actively working in the field of investigation of olfactory mucosa neural stem/progenitor and ensheathing cells. Development of methods for obtaining the cell cultures from the olfactory mucosa and the study of the therapeutic efficacy of these cells in experimental posttraumatic spinal cord cysts will create the preconditions for their successful application in the treatment of patients with posttraumatic cysts of spinal cord
Statement of the Problem: The pathological processes developing after spinal cord injuries often result in the formation of cysts. Treatment of posttraumatic spinal cord cysts is a difficult task, and the existing surgical and medical methods are ineffective. One of the most promising and emerging trends is cell therapy. The most perspective type of cells may be the ensheathing cells of the olfactory mucosa. However, the therapeutic effect of these cells on posttraumatic cysts of the spinal cord remains unexplored. The purpose of this research is to study the therapeutic effect of rat olfactory ensheathing cell transplantation on posttraumatic cysts of the spinal cord. Methodology & Theoretical Orientation: The ensheathing cells were obtained from rat olfactory mucosa by our modified procedure and were characterized by the expression of markers p75NTR and GFAP detected by immunofluorescence method. The study of the therapeutic effect of the transplantation on posttraumatic cysts was conducted on a model developed in our laboratory. The formation of cysts was confirmed by MRI. The cells were transplanted in different amounts (750000, 1500000). The dynamic of the recovery of motor activity of the hind limbs of rats was assessed using the BBB test. Findings: The improvement of the motor activity after transplantation of the olfactory ensheathing cells into posttraumatic cysts was demonstrated. The necessary number of cells (1.5 million) for the transplantation was also established, and a neuroprotective effect of a given number of cells was revealed. Conclusion & Significance: For the first time the therapeutic effect of rat olfactory ensheathing cell transplantation on posttraumatic cysts was demonstrated. Further research in this area will help to develop a combined use of the cell therapy by olfactory ensheathing cells with surgical and drug therapy for the treatment of patients with posttraumatic cysts of the spinal cord.
Hyo Jin Kang has his professional experiences in the Department of Neurosurgery, Seoul National University Hospital Clinical Research Institute (~2007), Department of Urology, Yonsei University Medical Center Research Center (~2015), Department of Biological Sciences, Yonsei University College of Medicine (~2017) and Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital (~2018). He has his teaching experiences in the Department of Life Science, Gachon University (2012 ~ 2014).
Chronic inflammation and impaired neovascularization are a critical factor contributing to delayed wound healing in diabetic patients. To overcome the therapeutic limitations of diabetic wounds (DBW), we investigated the effects of catechol-functionalized hyaluronic acid (HA-CA) patch combined with adipose-derived mesenchymal stem cells (ADSCs) in diabetic wound mouse models. The diabetic mice (DB, C57BL/6) were induced with streptozotocin (50 mg/kg, < 250 mg/dL) and divided into 4 groups; control (DBW), DBW+ADSCs, DBW+HA-CA, DBW+HA-CA+ADSCs (n=10 each). The ADSCs were labeled with fluorescent cell tracer PKH26 to detect the mesenchymal stem cells (MSCs) migration. The wound area was evaluated by ImageJ and was assessed for collagen content, thickness and vascularity of granulation tissue, apoptosis, re-epithelialization. The angiogenesis was evaluated by immunohistochemistry (von Willebrand Factor and CD31) and real-time PCR. The DBW healings were improved in all groups compared with control, especially in DBW+HA-CA+ADSCs group. In histopathologic scoring about edema, inflammation and congestion, the score significantly decreased in DBW+HA-CA+ADSCs group. PKH26-labeled ADSCs were observed in the subcutaneous tissue and muscle layer of the wounds. Apoptotic cells by TUNEL staining were decreased in all groups compared with control. In angiogenesis assay, the expressions of vWF and CD31 were significantly increased in DBW+HA-CA+ADSCs group. The HA-CA combined with ADSCs represents a promising approach to improve DBW healing by re-epithelialization and promoting angiogenesis. HA-CA combined with ADSCs will be a new strategy for clinical treatment.