Ion N. Mihailescu
National Institute for Lasers
Romania
Title: Soft transfer pulsed laser technologies for aplications in regenerative medicine
Biography
Biography: Ion N. Mihailescu
Abstract
Recent results in organic/inorganic composite nanostructured layers synthesized by MAPLE and LDW are reviewed. The optimum deposition regime was reached based upon the results of investigations by SEM, TEM, SAED, XTEM, AFM, XRD, XPS or FTIR methods. Biocompatibility, bioactivity and biodegradation were assessed by dedicated in-vitro tests. Urease immobilized by MAPLE in form of thin films was shown to preserve its activity in breaking down and diagnose of urea content in blood. The application of MAPLE was extended to the transfer and immobilization of IgG molecules. We studied the effect of the lipid addition in the initial solution upon the protein thin films adhesion to substrate. We showed that the composite PMMA-bioglass films efficiently protects metal implants against the action of human fluids. The MAPLE obtained nanocomposites Ag:HA-organosolv lignin proved noncytotoxic, supporting the normal development and promoting the proliferation of the adhered human mesenchymal cells. The lignin addition potentiated the anti-microbial activity of HA doped with silver ions against either bacterial or fungal biofilms. Mesotetraphenylporphyrin clean and liquid-free micropatterns on Si substrates were fabricated by LDW. The propulsor metal film thickness was found to be a key parameter, which determines the laser fluence range allowing the clean transfer, predominant mechanism of the blister formation and laser-induced heating of the transferred material. We conclude that the thin films prepared by MAPLE and LDW techniques were identical in chemical composition, structure, morphology, and most likely functionality resembling the base material, as proved by physical-chemical characterization and in-vitro assays.