Urszula Stachewicz
AGH University of Science and Technology
Poland
Title: 3D imaging of cell interactions with nanofibers scaffolds
Biography
Biography: Urszula Stachewicz
Abstract
The use of electrospun nanofibres for guided bone regeneration or bone scaffolds align with next-generation healthcare, especially as electrospun nanofibres are highlighted as being particularly effective in tissue engineering. However, optimization of the electrospinning process for cell growth and their interaction with nanofibre surfaces is yet to be determined. We develop a biodegradable electrospun nanofibre membrane for guided bone regeneration for bone scaffold applications. The interaction between osteoblasts and osteoblast-derived mineralized nodule formation on the nanofibre membrane is visualized using 3D imaging based on ‘slice-and-view’ combinations of ion beam and scanning electron microscopies (FIB-SEM). The presented 3D imaging technique therefore shows s a new approach in high resolution visualization the cell growth on electrospun nanofibers, and potentially other biomaterials, that will develop and design new biomaterials for a range of clinically important applications including orthopaedics of this work.