Vladimir Mironov
3D Bioprinting Solutions, Russia
Title: Scaffold-free, label-free and nozzle-free biofabrication technology based on magnetic levitational assembly
Biography
Biography: Vladimir Mironov
Abstract
Tissue spheroids have been proposed to use as building blocks in biofabrication and 3D bioprinting technologies. Label-based magnetic forces-driven 2D patterning of tissue spheroids requires cell labeling by magnetic nanoparticles. Recently novel label-free approach for magnetic levitational assembly has been introduced. Here we report a first time rapid assembly of 3D tissue engineered construct using scaffold-free and label-free magnetic levitation of tissue spheroids. Tissue spheroids (so-called chondrospheres) of standard size and shape capable of tissue fusion have been biofabricated using non-adhesive cell culture flasks from primary culture of ovine chondrocytes. Label-free magnetic levitation has been performed using experimental set with permanent magnets in presence of gadolinium in cell culture media which enables magnetic levitation. Potential toxic effect of gadolinium has been systematically evaluated. Mathematical modeling and computer simulations have been used for modeling of magnetic field and kinetics of tissue spheroids assembly into 3D tissue constructs. Plastic beads have been initially used as physical analogs of tissue spheroids for determining an optimal regime of magnetic levitation in presence of parmagnetic gadolinium medium. It has been shown that chondrospheres were able to rapidly assemble into 3D tissue construct in the permanent magnetic field in presence of gadolinium in cell culture media. Thus, label-free magnetic levitation of tissue spheroids represents a perspective approach for rapid scaffold-free 3D biofabrication and an attractive alternative to label-based magnetic tissue engineering.